Research Histology Services

Slide Preparation & Staining

What are these services for? RHS offers a wide array of special stains to visualize your tissue as a whole. If you are interested in looking at a specific antigen please see our Immunohistochemistry Services.

What does the customer provide? The customer would provide glass slides with tissue sections. For customers requesting immunohistochemistry services, customers must provide sufficient amounts of primary antibody. If you have samples that are embedding in blocks but need to be sectioned, please refer to our Sectioning Services page. If you have samples that need to be embedded into blocks, please refer to our Sample Preparation Services page.

What does RHS provide? RHS provides all of the reagents necessary for the staining of your tissues (except primary antibodies). RHS will stain your tissues, coverslip, and return the slides to you for imaging. Digital slide scanning is available.

H&E Stain on Blank Slide

  • What is Hematoxylin and Eosin? Hematoxylin and Eosin is the hallmark stain of pathology and is widely used to elucidate structures within the cell and the tissue.

Level I Special Stains to Elucidate Specific Cellular Characteristics

  • Luxol Blue (for myelin)
  • Masson’s Trichrome (for collagen and connective tissue)
  • Periodic Acid-Schiff [used to detect polysaccharides (i.e. glycogen), mucosubstances (i.e. glycoproteins, glycolipids) and mucins]
  • Periodic Acid-Schiff with digestion (for confirmation of glycogen)
  • Prussian Blue (for iron)
  • Picosirius red (for collagen I and III fibers)
  • Toluidine blue (for mast cells)
  • Safranin O (for cartilage)
  • Oil red O (for lipids)
  • Alcian blue (for mucins)
  • Verhoeff-Van Gieson (for elastic fibers)
  • Ask for availability of others!

Level II Special Stains to Elucidate Specific Cellular Characteristics

  • Silver Stains
  • Methenamine Silver Trichrome (for basement membrane)
  • Grocott's methenamine silver (for fungus)
  • Von Kossa (for calcium/mineralization)
  • Ask for availability of others

Apoptosis (TUNEL Staining)

  • What is TUNEL Staining for? Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the terminal end of nucleic acids that results from apoptotic signaling cascades. For detection of apoptotic cells, RHS laboratory utilizes the ApopTag® Peroxidase In Situ Apoptosis Detection Kit from Milliipore (S7100, manufacturer’s website).